Bioform solutions has developed a robust assay to monitor the cell health of probiotics, regardless of strains or the matrix. Flow cytometry is a single cell analysis tool. It can enumerate bacteria or yeast, alone or in a mix. It is the only way to enumerate heat killed cells (postbiotics) since they won’t grow. CFUs have been used in the industry for decades due to no extra equipment or knowledge needed, but the data has a 30-50% variability which leads to large questions about its accuracy. It also can only see cells that can grow in a particular media, so mixed strain probiotics can be very problematic. When the cells become stressed out due to processing and packaging, they can become viable but not culturable (VBNC), which means they won’t grow in a CFU assay. Flow cytometry gets around these issues by viewing all of the cells in a product, quickly and accurately with a CV <5%. Flow cytometry takes ~15 minutes whereas the CFU assay takes 3 days. This allows quick decisions to be made as a product is being formulated as well as determining the stability of the products.
We are a dynamic pre-clinical CRO offering the best-in-class in vitro simulations of the gastrointestinal tract to the food, functional food, feed and pharma industry. We offer a broad range of solutions for in vitro simulation of the gastrointestinal tract. Our technology platforms cover the physiology of the upper gastrointestinal tract, the physiology and the microbiology of the lower gastrointestinal tract and the microbiome-host interactions, and range from high-throughput screenings (Colon-on-a-plate®) to in-depth simulation of the long-term impact using the SHIME®.
By sharing our expertise and using our technology platforms, we assist your product development by building up essential know-how in the field of gastrointestinal transit, digestion, bioaccessibility, bioavailability, targeted delivery, drug dissolution, metabolism, microbial fermentation activity, ileal and colonic microbiome, gut barrier integrity, inflammation, immunomodulation and efficacy of probiotics, prebiotics, functional ingredients and pharmaceuticals, in relation to their fate and function in the body.
Recently we expanded our scope with a new business unit, MetaKey®, completely dedicated to metabolomics. In addition to classical UHPLC-HRMS-based untargeted and targeted metabolomics services, MetaKey® offers an innovative LA-REIMS platform for screening based on metabolic fingerprinting, which enables a high throughput at an affordable cost. Applications include analysis of any kind of matrix, but with a particular focus on microbial metabolism and interactions of microbiota with the human body.
A strong team, the desire to innovate and our customers’ satisfaction are at the core of our DNA. Our long-standing expertise and tailor-made solutions support your research from early phases to the final stages of product development. Our long-standing experience and expertise in studying the gastrointestinal processes at your service!
Human Metabolome Technologies (HMT) is a metabolomics profiling company that specializes in pre-clinical and clinical studies. HMT provides both targeted and untargeted profiling metabolite data for a wide range of applications from any sample type. HMT enables clients to understand critical pathways for biomarker discovery, enhance drug development, perform microbiome screening and uncover mechanisms of action through profiling of over 2,000 polar metabolites using high resolution, high performance CE-MS and LC-MS solutions for hundreds of lipophilic metabolites. HMT has been building a best in class metabolite profiling program with over 17 years of service to the research and medical community.